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inverted epifluorescence nikon ti e widefield microscope  (Nikon)


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    Structured Review

    Nikon inverted epifluorescence nikon ti e widefield microscope

    Inverted Epifluorescence Nikon Ti E Widefield Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 59676 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/widefield+inverted+epifluorescence+microscope+ti-e/Objectives/pmc07225751-536-23-25
    Average 99 stars, based on 59676 article reviews
    inverted epifluorescence nikon ti e widefield microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "Actin Assembly around the Shigella -Containing Vacuole Promotes Successful Infection"

    Article Title: Actin Assembly around the Shigella -Containing Vacuole Promotes Successful Infection

    Journal: Cell Reports

    doi: 10.1016/j.celrep.2020.107638


    Figure Legend Snippet:

    Techniques Used: Transduction, Plasmid Preparation, Mutagenesis, Expressing, Recombinant, Transfection, Electron Microscopy, Modification, Protease Inhibitor, Bicinchoninic Acid Protein Assay, Stable Transfection, Control, Software, Microscopy, Imaging, Cell Culture

    Related Articles

    Imaging:

    Article Title: A Transient Rise in Free Mg 2+ Ions Released from ATP-Mg Hydrolysis Contributes to Mitotic Chromosome Condensation.
    Article Snippet: .. Mg2+ imaging by NLS-MARIO in HeLa S3 cells HeLa S3 cells were cultured on a 35 mm glass-bottom dish containing 1.5 mL FluoroBrite DMEM (Thermo Fisher Scientific) supplemented with 10% FBS and imaged with a widefield inverted epifluorescence microscope (Ti-E, Nikon) equipped with a 40 3 oil immersion objective (Plan Fluor, numerical aperture [NA] 1.3; Nikon). .. The samples were illuminated with a 100 W mercury arc lamp through 5%, 12.5%, and 25% neutral density filters and a 434/17 excitation filter (Semrock).

    Cell Culture:

    Article Title: A Transient Rise in Free Mg 2+ Ions Released from ATP-Mg Hydrolysis Contributes to Mitotic Chromosome Condensation.
    Article Snippet: .. Mg2+ imaging by NLS-MARIO in HeLa S3 cells HeLa S3 cells were cultured on a 35 mm glass-bottom dish containing 1.5 mL FluoroBrite DMEM (Thermo Fisher Scientific) supplemented with 10% FBS and imaged with a widefield inverted epifluorescence microscope (Ti-E, Nikon) equipped with a 40 3 oil immersion objective (Plan Fluor, numerical aperture [NA] 1.3; Nikon). .. The samples were illuminated with a 100 W mercury arc lamp through 5%, 12.5%, and 25% neutral density filters and a 434/17 excitation filter (Semrock).

    Article Title: A Transient Rise in Free Mg 2+ Ions Released from ATP-Mg Hydrolysis Contributes to Mitotic Chromosome Condensation.
    Article Snippet: Retroviral particles, which were produced by lipofection of the Platinum-A retroviral packaging cell line (Cell Biolabs) with pQCXINAT1.03, were used to obtain a HeLa S3 cell line stably expressing ATeam. .. To image ATP in HeLa S3 cells expressing ATeam [32], cells were cultured on a 35 mm glass-bottom dish containing 2 mL phenolred-free DMEM (low glucose) supplemented with 10% FBS and imaged with a widefield inverted epifluorescence microscope (Ti-E, Nikon) equippedwith a 403dry objective (PlanApo, numerical aperture 0.95;Nikon). .. The sampleswere illuminatedwith a 75Wxenon arc lamp through 12.5% and 25% neutral density filters and a 438/24 excitation filter (Semrock).

    Inverted Epifluorescence:

    Article Title: A Transient Rise in Free Mg 2+ Ions Released from ATP-Mg Hydrolysis Contributes to Mitotic Chromosome Condensation.
    Article Snippet: .. Mg2+ imaging by NLS-MARIO in HeLa S3 cells HeLa S3 cells were cultured on a 35 mm glass-bottom dish containing 1.5 mL FluoroBrite DMEM (Thermo Fisher Scientific) supplemented with 10% FBS and imaged with a widefield inverted epifluorescence microscope (Ti-E, Nikon) equipped with a 40 3 oil immersion objective (Plan Fluor, numerical aperture [NA] 1.3; Nikon). .. The samples were illuminated with a 100 W mercury arc lamp through 5%, 12.5%, and 25% neutral density filters and a 434/17 excitation filter (Semrock).

    Article Title: Deconwolf enables high-performance deconvolution of widefield fluorescence microscopy images.
    Article Snippet: .. Quantification of smFISH dots in ×20 magnification images We imaged 10 field of view (FOVs) of a breast adenocarcinoma tissue core in a TMA on which we performed smFISH with a probe targeting MKI67 gene transcripts (Experimental Procedure), using a widefield inverted epifluorescence microscope (Nikon Ti-E) with a ×20 air objective (Nikon CFI Plan Apo VC ×20). .. For a subset of the regions imaged with the ×20 objective, we also acquired images using a ×60 oil objective (Nikon CFI Plan Apochromat Lambda 60XC).

    Article Title: Deconwolf enables high-performance deconvolution of widefield fluorescence microscopy images
    Article Snippet: .. We imaged 10 field of view (FOVs) of a breast adenocarcinoma tissue core in a TMA on which we performed smFISH with a probe targeting MKI67 gene transcripts (Experimental Procedure), using a widefield inverted epifluorescence microscope (Nikon Ti-E) with a ×20 air objective (Nikon CFI Plan Apo VC ×20). .. For a subset of the regions imaged with the ×20 objective, we also acquired images using a ×60 oil objective (Nikon CFI Plan Apochromat Lambda 60XC).

    Article Title: LC3 lipidation is essential for TFEB activation during the lysosomal damage response to kidney injury.
    Article Snippet: 1Department of Genetics, Graduate School of Medicine, Osaka University, Osaka, Japan.. 2Department of Intracellular Membrane Dynamics, Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan.. 3Institute for Advanced Co-Creation Studies, Osaka University, Osaka, Japan.

    Article Title: Actin Assembly around the Shigella -Containing Vacuole Promotes Successful Infection
    Article Snippet: In parallel, upscaled samples for western blot quantification were prepared in 6-well plates and for live imaging at the DeltaVision wide-field microscope in 35 mm glass bottom μ-Dishes (Ibidi, #81158) ( A–4C, C, 6D, A, and A). .. For quantitative screening experiments ( F, 1G, B, B–3D, D–4F, A, 5B, E–S1G, , , and B–S6D), image acquisition was performed using an inverted epifluorescence Nikon Ti-E widefield microscope with a 20x (0.5 Numerical Aperture (NA), 2.1 Working Distance (WD)) N-Plan air objective, an automatic programmable xy stage, and the Nikon perfect focus system. ..

    Article Title: A Transient Rise in Free Mg 2+ Ions Released from ATP-Mg Hydrolysis Contributes to Mitotic Chromosome Condensation.
    Article Snippet: Retroviral particles, which were produced by lipofection of the Platinum-A retroviral packaging cell line (Cell Biolabs) with pQCXINAT1.03, were used to obtain a HeLa S3 cell line stably expressing ATeam. .. To image ATP in HeLa S3 cells expressing ATeam [32], cells were cultured on a 35 mm glass-bottom dish containing 2 mL phenolred-free DMEM (low glucose) supplemented with 10% FBS and imaged with a widefield inverted epifluorescence microscope (Ti-E, Nikon) equippedwith a 403dry objective (PlanApo, numerical aperture 0.95;Nikon). .. The sampleswere illuminatedwith a 75Wxenon arc lamp through 12.5% and 25% neutral density filters and a 438/24 excitation filter (Semrock).

    Generated:

    Article Title: Single quantum dot imaging reveals PKCβ-dependent alterations in membrane diffusion and clustering of an ADHD/autism/bipolar disorder-associated dopamine transporter variant
    Article Snippet: At the microscope, IDT307 was added to the MatTek dish prior to imaging. .. Time-series images were generated by TIRF and widefield epifluorescence microscopy on a Nikon Eclipse Ti-E inverted microscope equipped with an Andor Zyla 4.2 PLUS sCMOS camera and viewed with an Apo TIRF 60×/1.49 NA oil objective. .. Excitation at 488 nm was sourced by a Nikon LU-NV laser unit.

    Epifluorescence Microscopy:

    Article Title: Single quantum dot imaging reveals PKCβ-dependent alterations in membrane diffusion and clustering of an ADHD/autism/bipolar disorder-associated dopamine transporter variant
    Article Snippet: At the microscope, IDT307 was added to the MatTek dish prior to imaging. .. Time-series images were generated by TIRF and widefield epifluorescence microscopy on a Nikon Eclipse Ti-E inverted microscope equipped with an Andor Zyla 4.2 PLUS sCMOS camera and viewed with an Apo TIRF 60×/1.49 NA oil objective. .. Excitation at 488 nm was sourced by a Nikon LU-NV laser unit.

    Article Title: Spatiotemporal kinetics of the SRP pathway in live E. coli cells
    Article Snippet: .. Widefield epifluorescence microscopy was performed on a Nikon Ti-E inverted microscope equipped with a CFI Apochromat TIRF 100XC Oil 1.49 NA objective (Nikon). .. Bright-field and fluorescence images were recorded with an EMCCD iXon 897 Ultra camera (Andor) connected through an additional magnifying 2.0× camera adapter (Diagnostic Instruments).

    Inverted Microscopy:

    Article Title: Single quantum dot imaging reveals PKCβ-dependent alterations in membrane diffusion and clustering of an ADHD/autism/bipolar disorder-associated dopamine transporter variant
    Article Snippet: At the microscope, IDT307 was added to the MatTek dish prior to imaging. .. Time-series images were generated by TIRF and widefield epifluorescence microscopy on a Nikon Eclipse Ti-E inverted microscope equipped with an Andor Zyla 4.2 PLUS sCMOS camera and viewed with an Apo TIRF 60×/1.49 NA oil objective. .. Excitation at 488 nm was sourced by a Nikon LU-NV laser unit.

    Article Title: Spatiotemporal kinetics of the SRP pathway in live E. coli cells
    Article Snippet: .. Widefield epifluorescence microscopy was performed on a Nikon Ti-E inverted microscope equipped with a CFI Apochromat TIRF 100XC Oil 1.49 NA objective (Nikon). .. Bright-field and fluorescence images were recorded with an EMCCD iXon 897 Ultra camera (Andor) connected through an additional magnifying 2.0× camera adapter (Diagnostic Instruments).

    Microscopy:

    Article Title: Actin Assembly around the Shigella -Containing Vacuole Promotes Successful Infection
    Article Snippet: In parallel, upscaled samples for western blot quantification were prepared in 6-well plates and for live imaging at the DeltaVision wide-field microscope in 35 mm glass bottom μ-Dishes (Ibidi, #81158) ( A–4C, C, 6D, A, and A). .. For quantitative screening experiments ( F, 1G, B, B–3D, D–4F, A, 5B, E–S1G, , , and B–S6D), image acquisition was performed using an inverted epifluorescence Nikon Ti-E widefield microscope with a 20x (0.5 Numerical Aperture (NA), 2.1 Working Distance (WD)) N-Plan air objective, an automatic programmable xy stage, and the Nikon perfect focus system. ..

    Expressing:

    Article Title: A Transient Rise in Free Mg 2+ Ions Released from ATP-Mg Hydrolysis Contributes to Mitotic Chromosome Condensation.
    Article Snippet: Retroviral particles, which were produced by lipofection of the Platinum-A retroviral packaging cell line (Cell Biolabs) with pQCXINAT1.03, were used to obtain a HeLa S3 cell line stably expressing ATeam. .. To image ATP in HeLa S3 cells expressing ATeam [32], cells were cultured on a 35 mm glass-bottom dish containing 2 mL phenolred-free DMEM (low glucose) supplemented with 10% FBS and imaged with a widefield inverted epifluorescence microscope (Ti-E, Nikon) equippedwith a 403dry objective (PlanApo, numerical aperture 0.95;Nikon). .. The sampleswere illuminatedwith a 75Wxenon arc lamp through 12.5% and 25% neutral density filters and a 438/24 excitation filter (Semrock).



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    Image Search Results


    Journal: Cell Reports

    Article Title: Actin Assembly around the Shigella -Containing Vacuole Promotes Successful Infection

    doi: 10.1016/j.celrep.2020.107638

    Figure Lengend Snippet:

    Article Snippet: For quantitative screening experiments ( F, 1G, B, B–3D, D–4F, A, 5B, E–S1G, , , and B–S6D), image acquisition was performed using an inverted epifluorescence Nikon Ti-E widefield microscope with a 20x (0.5 Numerical Aperture (NA), 2.1 Working Distance (WD)) N-Plan air objective, an automatic programmable xy stage, and the Nikon perfect focus system.

    Techniques: Transduction, Plasmid Preparation, Mutagenesis, Expressing, Recombinant, Transfection, Electron Microscopy, Modification, Protease Inhibitor, Bicinchoninic Acid Protein Assay, Stable Transfection, Control, Software, Microscopy, Imaging, Cell Culture